Human Molecular Genetics: Human Molecular Genetics
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Human Molecular Genetics: Human Molecular Genetics

SKU: 9780120443109

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Additional information

Full Title

Human Molecular Genetics: Human Molecular Genetics
Author(s)

AUTHOR, UNKNOWN
Edition
ISBN

9780120443109, 9780080536415
Publisher

Academic Press
Format

PDF and EPUB

Description

Human Molecular Genetics is a practical guide to the applications of molecular biology and genetics techniques to human cells. A wide range of experimental procedures for investigating human genes and genomes are presented.

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* Mutation Detection in Human Genes – chemical mismatch cleavage, DNA mini-sequencing, SSCP method, RT-PCR, electrophoretic mobility shift assay (EMSA), protein truncation test, chromosome deletion analysis.
* Gene Mapping, Cloning, Sequencing – gene linkage determination, large-capacity cloning system, cDNA isolation, differential display method, primer-based DNA sequencing.
* Transcription: Promoters, Transcription Factors, mRNA, – promotor mutation analysis, transcription factor identification, mRNA-protein interaction characterization.
* RNA Editing, Ribozymes, Antisense RNA-mammalian RNA editing assays, ribozymes as genetic tools, antisense RNA technology.
* Genome Recombination, Amplification – recombination assays for mammalian cells, gene amplification measurement.
* Receptors, Signal Transduction – intra-cellular receptor characterization, analysis of signal transduction genes.
* The Mouse as a Model System for Human Molecular Genetics – mouse genome methods (mouse crosses, somatic cell hybrids, YACs), mouse model for cardiovascular disease.

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