Celiac Disease 1st Edition
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Celiac Disease 1st Edition

SKU: 9780443192036

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Additional information

Full Title

Celiac Disease 1st Edition
Author(s)

Ainara Castellanos-Rubio
Edition

1st Edition
ISBN

9780443192036, 9780443192029
Publisher

Academic Press
Format

PDF and EPUB

Description

Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more.

Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.

  • Provides up-to-date and extensive protocols for the research on different aspects of celiac disease pathogenesis
  • Includes original figures which help users understand and follow the described protocols
  • Covers research on different aspects of disease, covering a wide range of technologies

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